rapid detection of vibrio cholerae by polymerase chain reaction based on

quick identification of vibrio cholerae in epidemics is   important, on the other hand; conventional methods are time-consuming and   costly. the aim of this study was to develop a rapid, inexpensive and high   sensitivity method for quick identification of vibrio cholerae . for   this purpose we designed a pcr detection based on magnetic nanoparticles for   identification of bacterial dna by pcr dynabead. so we used the biotinylated   probe for binding to dna extracted from vibrio cholera e and other bacterial   species ( salmonella, shigella, pseudomonas, e.coli ) using magnetic   bead isolated with magnetic field, the vibrio genome-specific primers (hlya)   for   pathogen detection (pcr) was used. the results showed specific band was just   for vibrio cholera (pcr positive); therefore designed probe was   specific for vibrio cholera e. according to the findings, this study is   characterized the high sensitivity of pcr using biotin-containing probes for   dna of vibrio cholera in contrast to the traditional methods.